multiple ihc assay kit tissuegnostics tgfp550 (TissueGnostics)
90
Structured Review
TissueGnostics
multiple ihc assay kit tissuegnostics tgfp550
Multiple Ihc Assay Kit Tissuegnostics Tgfp550, supplied by TissueGnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiple+ihc+assay+kit/pmc11991697-241-7-11?v=TissueGnostics
Average 90 stars, based on 1 article reviews
Multiple Ihc Assay Kit Tissuegnostics Tgfp550, supplied by TissueGnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiple+ihc+assay+kit/pmc11991697-241-7-11?v=TissueGnostics
Average 90 stars, based on 1 article reviews
multiple ihc assay kit tissuegnostics tgfp550 - by Bioz Stars,
2026-06
90/100 stars
Images
1) Product Images from "Loss function of tumor suppressor FRMD8 confers resistance to tamoxifen therapy via a dual mechanism"
Article Title: Loss function of tumor suppressor FRMD8 confers resistance to tamoxifen therapy via a dual mechanism
Journal: eLife
doi: 10.7554/eLife.101888
Figure Legend Snippet: ( A ) A diagram of the Frmd8 targeted alleles. Exons 4, 5, and 6 are flanked by loxp sites. ( B ) Distinguishing the genotype of littermate mice by mice coat color. Yellow represents MMTV-Cre + ; Frmd8 fl/fl ; PyMT genotype, and black represents MMTV-Cre - ; Frmd8 fl/fl ; PyMT genotype. ( C ) Representative PCR genotyping of mouse tail DNA. ( D ) Relative mRNA level of Frmd8 in 7-week-old mammary glands from PyMT mice was analyzed by qRT-PCR. Gapdh was used as an internal reference. *p<0.05 by unpaired Student’s t -test, n = 3. ( E ) Kaplan–Meier plot showing the appearance of palpable tumors in MMTV-Cre - ; Frmd8 fl/fl ; PyMT (n = 9) and MMTV-Cre + ; Frmd8 fl/fl ; PyMT (n = 9) mice (Log-rank test). ( F ) Representative images of tumors from MMTV-Cre - ; Frmd8 fl/fl ; PyMT and MMTV-Cre + ; Frmd8 fl/fl ; PyMT mice. ( G, H ) Total tumor weight ( G ) and number of tumors ( H ) per mice were measured. **p<0.01, ***p<0.001 by unpaired Student’s t -test. ( I ) Representative H&E staining of tumors from MMTV-Cre - ; Frmd8 fl/fl ; PyMT and MMTV-Cre + ; Frmd8 fl/fl ; PyMT mice. Scale bar, 50 μm. ( J ) Immunohistochemistry (IHC) staining for Ki67 expression in mammary tumors from PyMT mice. The black boxes represent the magnified typical staining of the original images. Scale bar, 50 μm. ( K ) Quantification of Ki67-positive cell percentage in ( J ). *p<0.05 by unpaired Student’s t -test, n = 3. ( L ) Kaplan–Meier plot showing the appearance of palpable tumors in MMTV-Cre - ; Frmd8 fl/fl ; PyMT and MMTV-Cre + ; Frmd8 fl/fl ; PyMT mice, with or without tamoxifen treatment (Log-rank test, *p<0.05, ***p<0.001, ****p<0.0001). ( M ) Representative images of tumors from MMTV-Cre - ; Frmd8 fl/fl ; PyMT and MMTV-Cre + ; Frmd8 fl/fl ; PyMT mice, with or without tamoxifen treatment. ( N, O ) Total tumor weight ( N ) and number of tumors ( O ) per mice from ( L ) were measured (n ≥ 10). *p<0.05, **p<0.01, ***p<0.001 by unpaired Student’s t -test or Mann–Whitney test. Figure 1—source data 1. Unedited gel pictures for , indicating the relevant bands. Figure 1—source data 2. Original files for gel pictures displayed in .
Techniques Used: Quantitative RT-PCR, Staining, Immunohistochemistry, Expressing, MANN-WHITNEY
Figure Legend Snippet: ( A–D ) The scatter plot shows the cells in multiplex immunofluorescence images of normal tissues adjacent to tumor in MMTV-Cre - ; Frmd8 fl/fl ; PyMT and MMTV-Cre + ; Frmd8 fl/fl ; PyMT mice. ( E ) Immunohistochemistry (IHC) staining for Frmd8 and ERα expression in 7-week-old mammary glands from PyMT mice. The black boxes represent the magnified typical staining of the original images. Scale bar, 100 μm.
Techniques Used: Multiplex Assay, Immunofluorescence, Immunohistochemistry, Expressing, Staining
